Towards high–throughput analyses of fecal samples from wildlife
نویسندگان
چکیده
منابع مشابه
Collecting Fecal Samples for Microbiome Analyses in Epidemiology Studies.
BACKGROUND The need to develop valid methods for sampling and analyzing fecal specimens for microbiome studies is increasingly important, especially for large population studies. METHODS Some of the most important attributes of any sampling method are reproducibility, stability, and accuracy. We compared seven fecal sampling methods [no additive, RNAlater, 70% ethanol, EDTA, dry swab, and pre...
متن کاملSurveillance of Foodborne Pathogens: Towards Diagnostic Metagenomics of Fecal Samples
Diagnostic metagenomics is a rapidly evolving laboratory tool for culture-independent tracing of foodborne pathogens. The method has the potential to become a generic platform for detection of most pathogens and many sample types. Today, however, it is still at an early and experimental stage. Studies show that metagenomic methods, from sample storage and DNA extraction to library preparation a...
متن کاملMolecular Identification of Giardia intestinalis and Blastocystis hominis Genotypes from Fecal Samples in Khuzestan Province, Iran
Background and purpose: Giardia and Blastocystis are common parasites of humans with a wide range of hosts. The aim of this study was to identify the genotypes of Giardia intestinalis and Blastocystis hominis in individuals attending Bavi health centers in Khuzestan province, Iran. Materials and methods: In this study, 30 positive stool samples of Giardia and Blastocystis were collected from i...
متن کاملComparison of Six Culture Methods for Salmonella Isolation from Poultry Fecal Samples
Abstract Background and Objective: Salmonellosis is one of the most important food-borne bacterial zoonotic diseases worldwide, and poultry and its products are the major sources for salmonella transmission to human. Isolation of Salmonellaenterica from poultry needs bacteriologic enrichment and selected cultures of fecal samples. In this study, different culture methods for the isolation of s...
متن کاملA novel affordable reagent for room temperature storage and transport of fecal samples for metagenomic analyses
BACKGROUND The number of large-scale studies on the gut microbiota in human cohorts is rapidly increasing. However, the few and expensive options for storage of fecal samples at room temperature have been an obstacle for large-scale metagenomic studies and the development of clinical/commercial personal metagenomic sequencing. RESULTS In this study, we systematically tested a novel N-octylpyr...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Animal Biodiversity and Conservation
سال: 2020
ISSN: 2014-928X,1578-665X
DOI: 10.32800/abc.2020.43.0271